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KMID : 0613820110210030468
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Journal of Life Science
2011 Volume.21 No. 3 p.468 ~ p.472
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Genetic Analysis of Polymorphic DNA Markers in Cucumber
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Lee Sun-Young
Chung Sang-Min
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Abstract
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DNA marker is a powerful tool for plant genetics and breeding. In this study, 995 SSR markers were employed with chilling resistant cucumber, known as ‘NC76’, and chilling susceptible cucumber, known as ‘GY14’. Using 2% agarose gel electrophoresis, 145 SSR markers were identified as length variation markers between ‘NC76’ and ‘GY14’. The SSR markers that showed no length polymorphism were then screened using high resolution melting analysis technique and additional 30 polymorphic SSR markers were identified. As a preliminary evaluation for mapping, 20 markers among these 175 markers were employed to a F₂ population of ‘NC76’ x ‘GY14’ cross. Linkage analysis revealed 13 markers that joined into six linkage groups and seven markers that remained unlinked. This result indicates that these 175 markers could be used for construction of a genetic map using a cross between ‘NC76’ and ‘GY14’ for further investigation in developing markers related to resistance to chilling in cucumbers.
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KEYWORD
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Simple sequence repeat (SSR), high resolution melting analysis (HRM), DNA marker, cucumber
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